Registro sencillo
dc.contributor |
Universitat de Vic. Escola Politècnica Superior |
|
dc.contributor |
Universitat de Vic. Grup de Recerca en Bioinformàtica i Estadística Mèdica |
|
dc.contributor.author |
De Arquer, Guillermo Robert
|
|
dc.contributor.author |
Peña, Ruth
|
|
dc.contributor.author |
Cabrera, Cecilia
|
|
dc.contributor.author |
Coma, Gemma
|
|
dc.contributor.author |
Ruiz Hernández, Raul
|
|
dc.contributor.author |
Guerola, Rosa
|
|
dc.contributor.author |
Clotet, Bonaventura
|
|
dc.contributor.author |
Ruiz, Lídia
|
|
dc.contributor.author |
Esté, José A.
|
|
dc.contributor.author |
Calle, M. Luz
|
|
dc.contributor.author |
Bofill, Margarita
|
|
dc.date.accessioned |
2012-10-17T08:21:32Z |
|
dc.date.available |
2012-10-17T08:21:32Z |
|
dc.date.created |
2007 |
|
dc.date.issued |
2007 |
|
dc.identifier.citation |
DE ARQUER, Guillermo Roberto i altres . "Skewed expression and up-regulation of the IL-12 and IL-18 receptors in resting and activated CD4 T cells from HIV-1-infected patients". A: Journal of leukocyte biology, june 29, 2007, vol. 82, núm. 1, pàg. 72-78. doi:10.1189/jlb.1106698 |
ca_ES |
dc.identifier.issn |
0741-5400 |
|
dc.identifier.uri |
http://hdl.handle.net/10854/1903 |
|
dc.description.abstract |
IL-12 and IL-18 synergistically induce
the production of IFN- by resting and activated T
cells. To evaluate whether this induction was affected
in HIV-1-infected patients, PBMC or isolated
CD4 T cells were cultured with IL-12 plus
IL-18, anti-CD3 plus anti-CD28, or PHA for 72 h.
Cell samples were labeled daily to assess the levels
of IL-12 receptor 1 (IL-12R 1), IL-12R 2, and
IL-18R . Culture supernatants were analyzed for
the presence of Th1- and Th2-related cytokines by
ELISA or cytometric bead array and analyzed by
flow cytometry. A twofold increase in the percentage
of CD4-resting T cells expressing IL-12R 1
and IL-18R from HIV-1-infected patients was observed
when compared with cells from HIV-1-negative
donors. Higher IL-12R 1 and IL-18R expression
correlated (r 0.87; P<0.007) to increased
production of IFN- by isolated CD4 T
cells in the presence of IL-12 and IL-18. Moreover,
exogenous IL-12 and IL-18 induced the upregulation
of IL-12R 2 to twice higher in CD4 T
cells from HIV-1-positive individuals compared
with controls. Conversely, upon activation with
anti-CD3 and anti-CD28 antibodies, only 25% of
the CD4 T cells from HIV-1 patients showed an
increase in the IL-12 2 when compared with 50%
in healthy controls. Furthermore, the percentage
of IL-12R 1-positive cells correlated inversely
with the CD4 nadir of patients, suggesting that
deregulation of the IL-12 and IL-18 pathways may
play a role in the immunopathogenesis of HIV-1
infection. J. Leukoc. Biol. 82: 72–78; 2007. |
ca_ES |
dc.format |
application/pdf |
|
dc.format.extent |
7 p. |
ca_ES |
dc.language.iso |
eng |
ca_ES |
dc.publisher |
Society for Leukocyte Biology |
ca_ES |
dc.rights |
(c) Society for Leukocyte Biology |
|
dc.rights |
Tots els drets reservats |
ca_ES |
dc.subject.other |
Bioinformàtica |
ca_ES |
dc.title |
Skewed expression and up-regulation of the IL-12 and IL-18 receptors in resting and activated CD4 T cells from HIV-1-infected patients |
ca_ES |
dc.type |
info:eu-repo/semantics/article |
ca_ES |
dc.identifier.doi |
https://doi.org/doi:10.1189/jlb.1106698 |
|
dc.relation.publisherversion |
http://dx.doi.org/doi:10.1189/jlb.1106698 |
|
dc.rights.accessRights |
info:eu-repo/semantics/closedAccess |
|
dc.type.version |
info:eu-repo/semantics/publishedVersion |
|
dc.indexacio |
Indexat a SCOPUS |
|
dc.indexacio |
Indexat a WOS/JCR |
ca_ES |
Texto completo de este documento
Registro sencillo