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A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm)

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dc.contributor Universitat de Vic - Universitat Central de Catalunya. Departament de Biologia de Sistemes
dc.contributor.author Sanchez Calle, Anna
dc.contributor.author Nair, Neha
dc.contributor.author KoKo Oo, Aung
dc.contributor.author Prieto-Vila, Marta
dc.contributor.author Koga, Megumi
dc.contributor.author Cahya Khayrani, Apriliana
dc.contributor.author Hussein, Maram
dc.contributor.author Hurley, Laura
dc.contributor.author Vaidyanath, Arun
dc.contributor.author Seno, Akimasa
dc.contributor.author Iwasaki, Yoshiaki
dc.contributor.author Calle, M. Luz
dc.contributor.author Kasai, Tomonari
dc.contributor.author Seno, Masaharu
dc.date.accessioned 2017-06-22T16:15:06Z
dc.date.available 2017-06-22T16:15:06Z
dc.date.created 2016
dc.date.issued 2016
dc.identifier.citation Calle, A. S., Nair, N., Oo, A. K., Prieto-Vila, M., Koga, M., Khayrani, A. C., et al. (2016). A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm). American Journal of Cancer Research, 6(12), 2799-2815. es
dc.identifier.issn 2156-6976
dc.identifier.uri http://hdl.handle.net/10854/5027
dc.description.abstract Pancreatic ductal adenocarcinoma (PDAC) is the most representative form of pancreatic cancers. PDAC solid tumours are constituted of heterogeneous populations of cells including cancer stem cells (CSCs), differentiated cancer cells, desmoplastic stroma and immune cells. The identification and consequent isolation of pancreatic CSCs facilitated the generation of genetically engineered murine models. Nonetheless, the current models may not be representative for the spontaneous tumour occurrence. In the present study, we show the generation of a novel pancreatic iPSC-converted cancer stem cell lines (CSCcm) as a cutting-edge model for the study of PDAC. The CSCcm lines were achieved only by the influence of pancreatic cancer cell lines conditioned medium and were not subjected to any genetic manipulation. The xenografts tumours from CSCcm lines displayed histopathological features of ADM, PanIN and PDAC lesions. Further molecular characterization from RNA-sequencing analysis highlighted primary culture cell lines (1st CSCcm) as potential candidates to represent the pancreatic CSCs and indicated the establishment of the pancreatic cancer molecular pattern in their subsequent progenies 2nd CSCcm and 3rd CSCcm. In addition, preliminary RNA-seq SNPs analysis showed that the distinct CSCcm lines did not harbour single point mutations for the oncogene Kras codon 12 or 13. Therefore, PDAC-CSCcm model may provide new insights about the actual occurrence of the pancreatic cancer leading to develop different approaches to target CSCs and abrogate the progression of this fatidic disease. es
dc.format application/pdf
dc.format.extent 17 p. es
dc.language.iso eng es
dc.publisher eCentury Publishing Corporation es
dc.rights Aquest document està subjecte a aquesta llicència Creative Commons es
dc.rights.uri http://creativecommons.org/licenses/by-nc/4.0/ es
dc.rights.uri http://www.ajtr.org/guidelines.html
dc.subject.other Càncer es
dc.subject.other Cèl·lules mare es
dc.subject.other Pàncrees -- Càncer es
dc.title A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm) es
dc.type info:eu-repo/semantics/article es
dc.rights.accesRights info:eu-repo/semantics/openAccess es
dc.type.version info:eu-repo/publishedVersion es
dc.indexacio Indexat a WOS/JCR es
dc.indexacio Indexat a SCOPUS es

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