RNA-sequencing for detecting changes between expression
patterns has emerged as a frequent tool in life sciences stud-
ies. However, it is an under-development tool which still
lacks a standard procedure. This way, many software pack-
ages and approaches could be used when designing an ex-
periment. Here we develop and present ...»»»»
RNA-sequencing for detecting changes between expression
patterns has emerged as a frequent tool in life sciences stud-
ies. However, it is an under-development tool which still
lacks a standard procedure. This way, many software pack-
ages and approaches could be used when designing an ex-
periment. Here we develop and present an approach based
on a scoring function. It allows using multiple packages and
relies its e ciency on validated data. Thus, the function
is adjusted using qPCR veri ed data. Then it is tested on
experimental data obtained from a Lolium Perenne drought
stress tolerance study yielding positive results with several
potential uses in further studies.^^^^