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dc.contributor |
Universitat de Vic - Universitat Central de Catalunya. Càtedra de la Sida i Malalties Relacionades |
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dc.contributor.author |
Molinos-Albert, Luis M.
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dc.contributor.author |
Carrillo, Jorge
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Curriu, Marta
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Rodríguez de la Concepción, Maria L.
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Marfil, Sílvia
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dc.contributor.author |
García, Elisabet
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Clotet, Bonaventura
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dc.contributor.author |
Blanco, Julià
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dc.date.accessioned |
2014-07-24T07:40:44Z |
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dc.date.available |
2014-07-24T07:40:44Z |
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dc.date.created |
2014 |
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dc.date.issued |
2014 |
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dc.identifier.citation |
Molinos-Albert, L. M., Carrillo, J., Curriu, M., Rodriguez de la Concepcion,Maria L., Marfil, S., Garcia, E., et al. (2014). Anti-MPER antibodies with heterogeneous neutralization capacity are detectable in most untreated HIV-1 infected individuals. Retrovirology, 11, 44. |
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dc.identifier.issn |
1742-4690 |
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dc.identifier.issn |
http://dx.doi.org/10.1186/1742-4690-11-44 |
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dc.identifier.uri |
http://hdl.handle.net/10854/3232 |
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dc.description.abstract |
Background
The MPER region of the HIV-1 envelope glycoprotein gp41 is targeted by broadly neutralizing antibodies. However, the localization of this epitope in a hydrophobic environment seems to hamper the elicitation of these antibodies in HIV infected individuals.
We have quantified and characterized anti-MPER antibodies by ELISA and by flow cytometry using a collection of mini gp41-derived proteins expressed on the surface of 293T cells. Longitudinal plasma samples from 35 HIV-1 infected individuals were assayed for MPER recognition and MPER-dependent neutralizing capacity using HIV-2 viruses engrafted with HIV-1 MPER sequences.
Results
Miniproteins devoid of the cysteine loop of gp41 exposed the MPER on 293T cell membrane. Anti-MPER antibodies were identified in most individuals and were stable when analyzed in longitudinal samples. The magnitude of the responses was strongly correlated with the global response to the HIV-1 envelope glycoprotein, suggesting no specific limitation for anti-MPER antibodies. Peptide mapping showed poor recognition of the C-terminal MPER moiety and a wide presence of antibodies against the 2F5 epitope. However, antibody titers failed to correlate with 2F5-blocking activity and, more importantly, with the specific neutralization of HIV-2 chimeric viruses bearing the HIV-1 MPER sequence; suggesting a strong functional heterogeneity in anti-MPER humoral responses.
Conclusions
Anti-MPER antibodies can be detected in the vast majority of HIV-1 infected individuals and are generated in the context of the global anti-Env response. However, the neutralizing capacity is heterogeneous suggesting that eliciting neutralizing anti-MPER antibodies by immunization might require refinement of immunogens to skip nonneutralizing responses. |
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dc.format |
application/pdf |
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dc.format.extent |
12 p. |
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dc.language.iso |
eng |
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dc.publisher |
Biomed central |
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dc.rights |
Aquest document està subjecte a aquesta llicència Creative Commons |
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dc.rights.uri |
http://creativecommons.org/licenses/by/3.0/es/ |
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dc.subject.other |
Sida -- Tractament |
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dc.title |
Anti-MPER antibodies with heterogeneous neutralization capacity are detectable in most untreated HIV-1 infected individuals |
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dc.type |
info:eu-repo/semantics/article |
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dc.relation.publisherversion |
http://www.retrovirology.com/content/11/1/44 |
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dc.rights.accessRights |
info:eu-repo/semantics/openAccess |
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dc.type.version |
info:eu-repo/publishedVersion |
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dc.indexacio |
Indexat a WOS/JCR |
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