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Detection of HIV-1-specific T-cell immune responses in highly HIV-exposed uninfected individuals by in-vitro dendritic cell co-culture

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dc.contributor Universitat de Vic - Universitat Central de Catalunya. Càtedra de la Sida i Malalties Relacionades
dc.contributor.author Guardo, Alberto C.
dc.contributor.author Ruiz-Riol, Marta
dc.contributor.author Fernández, Emma
dc.contributor.author Maleno, Maria J.
dc.contributor.author Bargalló, Manel E.
dc.contributor.author Leon, Agathe
dc.contributor.author Climent, Núria
dc.contributor.author García, Felipe
dc.contributor.author Gatell, J.M.
dc.contributor.author Brander, Christian
dc.contributor.author Plana, Montserrat
dc.date.accessioned 2015-11-13T09:59:44Z
dc.date.available 2015-11-13T09:59:44Z
dc.date.created 2015
dc.date.issued 2015
dc.identifier.citation Guardo, A. C., Ruiz-Riol, M., Fernández, E., Maleno, M. J., Bargalló, M. E., León, A., et al. (2015). Detection of HIV-1-specific T-cell immune responses in highly HIV-exposed uninfected individuals by in-vitro dendritic cell co-culture. Aids, 29(11), 1309-1318. ca_ES
dc.identifier.issn 0269-9370
dc.identifier.uri http://hdl.handle.net/10854/4350
dc.description.abstract Background: Although virus-specific responses are rarely detected by conventional approaches, we report here the detection of T-cell responses in HIV-exposed seronegative (HESN) patients by two distinct assays. Methods: HIV-specific T-cell responses were analyzed by enzyme-linked immunospot in peripheral blood mononuclear cells from HESN patients after a 48-h co-culture with boosted dendritic cells. Additionally, a boosted flow cytometry approach was used to capture antiviral T-cell responses. Host genetic factors and T-cell activation were also analyzed to assess their implication on HIV exposure. Results: Of the 45 HESN individuals tested, up to 11 (24.4%) showed at least one response to peptide pools covering HIV Gag and Nef. A positive correlation was observed between the intensity (P¼0.0022) and magnitude (P¼0.0174) of the response detected in the HESN, and the viral load of the HIV-positive partner. Moreover, the result from the boosted flow and cytomix analyses showed a dominant Th1- like response pattern against HIV antigens, especially in CD8þ T-cell populations. Conclusions: The combined use of our boosted dendritic cell technique with a boosted flow cytometric approach allows us both to detect specific HIV-positive responses in a higher percentage of HESN patients and to define specific effector function profiles. This study contributes to a better understanding of resistance to HIV infection. ca_ES
dc.format application/pdf
dc.format.extent 10 p. ca_ES
dc.language.iso eng ca_ES
dc.publisher Wolters Kluwer Health ca_ES
dc.rights Tots els drets reservats ca_ES
dc.rights (c) Wolters Kluwer Health, 2015
dc.subject.other Sida -- Tractament ca_ES
dc.subject.other VIH (Virus) ca_ES
dc.title Detection of HIV-1-specific T-cell immune responses in highly HIV-exposed uninfected individuals by in-vitro dendritic cell co-culture ca_ES
dc.type info:eu-repo/semantics/article ca_ES
dc.identifier.doi https://doi.org/10.1097/QAD.0000000000000728
dc.rights.accesRights info:eu-repo/semantics/closedAccess ca_ES
dc.type.version info:eu-repo/publishedVersion ca_ES
dc.indexacio Indexat a WOS/JCR ca_ES
dc.indexacio Indexat a SCOPUS ca_ES

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