Understanding the regulatory machinery is one of the current
challenges for research in cancer epigenomics. Regulation
of gene expression is a complex process with many components
involved in it such as histone modifications, DNA methylation
and chromatin accessibility, among others. In this project we are
going to explore ...»»»»
Understanding the regulatory machinery is one of the current
challenges for research in cancer epigenomics. Regulation
of gene expression is a complex process with many components
involved in it such as histone modifications, DNA methylation
and chromatin accessibility, among others. In this project we are
going to explore the Blueprint database of human hematopoietic
cells in order to characterize regulatory regions on samples
from this dataset. For this purpose we are going to carry out a
number of steps to analyse the DNA methylation, epigenomic
features and RNA-seq.
The characterization of methylation across 92 samples of 12
different cell types have determined that methylation state at
promoters and CpG islands are cell type-specific. Exploration
and integration of epigenomics in one sample of cell type monocyte
was carried out. We have seen the implications of two
marks studied (histone modification H3K4me3 and open chromatin
state) in gene expression. This characterization of promoters
functionality enabled us to create a pipeline for the identification
of novel regulatory regions based in the integration of
epigenomic features and RNA-seq data, which we later reproduced
in four samples. This project could be a start point of
a bigger project for the cell type-specific promoters discovery
within the Blueprint projects.^^^^