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dc.contributor |
Universitat de Vic - Universitat Central de Catalunya. Càtedra de la Sida i Malalties Relacionades |
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dc.contributor.author |
Guardo, Alberto C.
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dc.contributor.author |
Ruiz-Riol, Marta
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dc.contributor.author |
Fernández, Emma
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dc.contributor.author |
Maleno, Maria J.
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dc.contributor.author |
Bargalló, Manel E.
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Leon, Agathe
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Climent, Núria
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García, Felipe
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Gatell, J.M.
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Brander, Christian
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dc.contributor.author |
Plana, Montserrat
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dc.date.accessioned |
2015-11-13T09:59:44Z |
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dc.date.available |
2015-11-13T09:59:44Z |
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dc.date.created |
2015 |
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dc.date.issued |
2015 |
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dc.identifier.citation |
Guardo, A. C., Ruiz-Riol, M., Fernández, E., Maleno, M. J., Bargalló, M. E., León, A., et al. (2015). Detection of HIV-1-specific T-cell immune responses in highly HIV-exposed uninfected individuals by in-vitro dendritic cell co-culture. Aids, 29(11), 1309-1318. |
ca_ES |
dc.identifier.issn |
0269-9370 |
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dc.identifier.uri |
http://hdl.handle.net/10854/4350 |
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dc.description.abstract |
Background: Although virus-specific responses are rarely detected by conventional
approaches, we report here the detection of T-cell responses in HIV-exposed seronegative
(HESN) patients by two distinct assays.
Methods: HIV-specific T-cell responses were analyzed by enzyme-linked immunospot
in peripheral blood mononuclear cells from HESN patients after a 48-h co-culture with
boosted dendritic cells. Additionally, a boosted flow cytometry approach was used to
capture antiviral T-cell responses. Host genetic factors and T-cell activation were also
analyzed to assess their implication on HIV exposure.
Results: Of the 45 HESN individuals tested, up to 11 (24.4%) showed at least one
response to peptide pools covering HIV Gag and Nef. A positive correlation was
observed between the intensity (P¼0.0022) and magnitude (P¼0.0174) of the
response detected in the HESN, and the viral load of the HIV-positive partner. Moreover,
the result from the boosted flow and cytomix analyses showed a dominant Th1-
like response pattern against HIV antigens, especially in CD8þ T-cell populations.
Conclusions: The combined use of our boosted dendritic cell technique with a boosted
flow cytometric approach allows us both to detect specific HIV-positive responses in a
higher percentage of HESN patients and to define specific effector function profiles. This
study contributes to a better understanding of resistance to HIV infection. |
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dc.format |
application/pdf |
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dc.format.extent |
10 p. |
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dc.language.iso |
eng |
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dc.publisher |
Wolters Kluwer Health |
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dc.rights |
Tots els drets reservats |
ca_ES |
dc.rights |
(c) Wolters Kluwer Health, 2015 |
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dc.subject.other |
Sida -- Tractament |
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dc.subject.other |
VIH (Virus) |
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dc.title |
Detection of HIV-1-specific T-cell immune responses in highly HIV-exposed uninfected individuals by in-vitro dendritic cell co-culture |
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dc.type |
info:eu-repo/semantics/article |
ca_ES |
dc.identifier.doi |
https://doi.org/10.1097/QAD.0000000000000728 |
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dc.rights.accessRights |
info:eu-repo/semantics/closedAccess |
ca_ES |
dc.type.version |
info:eu-repo/publishedVersion |
ca_ES |
dc.indexacio |
Indexat a WOS/JCR |
ca_ES |
dc.indexacio |
Indexat a SCOPUS |
ca_ES |
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