DSpace Repository

Lama2 lentiviral payload. Directing a treatment for muscular dystrophy

Show simple item record

dc.contributor Universitat de Vic - Universitat Central de Catalunya. Facultat de Ciències i Tecnologia
dc.contributor.author Rivero Dacruz, Andrea
dc.date.accessioned 2019-07-16T10:40:21Z
dc.date.available 2019-07-16T10:40:21Z
dc.date.created 2019-06
dc.date.issued 2019-06
dc.identifier.uri http://hdl.handle.net/10854/5855
dc.description Curs 2018-2019 es
dc.description.abstract In this degree final project will be summarized the results from one of the experimental procedures performed in the Translational Synthetic Biology Department in the PRBB-UPF, a research group which has a project focused on finding a treatment for merosin-deficient congenital muscular dystrophy (MCD1A) disease, a variation of congenital muscular dystrophy (CMD) which may be caused by mutations in LAMA2 gene. These mutations may provoke a deficiency or a functional reduction of laminin α2 subunit, the heavy chain of Laminin-211 molecule, also known as merosin, a cell-adhesion molecule that is highly expressed in the basement membrane on skeletal muscle and protects the muscle fiber from damage because of the constant stress of muscle contractions. We aim to explore a curative treatment for Lama2 patients using animal models to explore its efficacy. To achieve this purpose, we will improve the expression of mice Lama2 gene in bone marrow cells by using lentiviral vectors to treat muscular dystrophy. To carry out this process we cloned Lama2 gene into lentiviral vector for this way infect mammal cells with lentivirus and, by extracting RNA, DNA and proteins from those cells, check the lentiviral titer with quantitative PCR assay to analyse its infective capacity. The results of the project have shown that despite the huge length Lama2 gene it’s possible to clone into lentiviral vectors and then by lentivirus infection, transfer the transgene to mammalian cells so that it produces functional proteins in a ratio that can be used to treat the disease. es
dc.format application/pdf es
dc.format.extent 46 p. es
dc.language.iso eng es
dc.rights Aquest document està subjecte a aquesta llicència Creative Commons es
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ca es
dc.subject.other Distròfia muscular -- Tractament es
dc.title Lama2 lentiviral payload. Directing a treatment for muscular dystrophy es
dc.type info:eu-repo/semantics/bachelorThesis es
dc.rights.accesRights info:eu-repo/semantics/openAccess es

Files in this item

Show simple item record

Aquest document està subjecte a aquesta llicència Creative Commons Except where otherwise noted, this item's license is described as Aquest document està subjecte a aquesta llicència Creative Commons

Search RIUVic


Browse

Statistics