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Anti-MPER antibodies with heterogeneous neutralization capacity are detectable in most untreated HIV-1 infected individuals

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dc.contributor Universitat de Vic - Universitat Central de Catalunya. Càtedra de la Sida i Malalties Relacionades
dc.contributor.author Molinos-Albert, Luis M.
dc.contributor.author Carrillo, Jorge
dc.contributor.author Curriu, Marta
dc.contributor.author Rodríguez de la Concepción, Maria L.
dc.contributor.author Marfil, Sílvia
dc.contributor.author García, Elisabet
dc.contributor.author Clotet, Bonaventura
dc.contributor.author Blanco, Julià
dc.date.accessioned 2014-07-24T07:40:44Z
dc.date.available 2014-07-24T07:40:44Z
dc.date.created 2014
dc.date.issued 2014
dc.identifier.citation Molinos-Albert, L. M., Carrillo, J., Curriu, M., Rodriguez de la Concepcion,Maria L., Marfil, S., Garcia, E., et al. (2014). Anti-MPER antibodies with heterogeneous neutralization capacity are detectable in most untreated HIV-1 infected individuals. Retrovirology, 11, 44. ca_ES
dc.identifier.issn 1742-4690
dc.identifier.issn http://dx.doi.org/10.1186/1742-4690-11-44
dc.identifier.uri http://hdl.handle.net/10854/3232
dc.description.abstract Background The MPER region of the HIV-1 envelope glycoprotein gp41 is targeted by broadly neutralizing antibodies. However, the localization of this epitope in a hydrophobic environment seems to hamper the elicitation of these antibodies in HIV infected individuals. We have quantified and characterized anti-MPER antibodies by ELISA and by flow cytometry using a collection of mini gp41-derived proteins expressed on the surface of 293T cells. Longitudinal plasma samples from 35 HIV-1 infected individuals were assayed for MPER recognition and MPER-dependent neutralizing capacity using HIV-2 viruses engrafted with HIV-1 MPER sequences. Results Miniproteins devoid of the cysteine loop of gp41 exposed the MPER on 293T cell membrane. Anti-MPER antibodies were identified in most individuals and were stable when analyzed in longitudinal samples. The magnitude of the responses was strongly correlated with the global response to the HIV-1 envelope glycoprotein, suggesting no specific limitation for anti-MPER antibodies. Peptide mapping showed poor recognition of the C-terminal MPER moiety and a wide presence of antibodies against the 2F5 epitope. However, antibody titers failed to correlate with 2F5-blocking activity and, more importantly, with the specific neutralization of HIV-2 chimeric viruses bearing the HIV-1 MPER sequence; suggesting a strong functional heterogeneity in anti-MPER humoral responses. Conclusions Anti-MPER antibodies can be detected in the vast majority of HIV-1 infected individuals and are generated in the context of the global anti-Env response. However, the neutralizing capacity is heterogeneous suggesting that eliciting neutralizing anti-MPER antibodies by immunization might require refinement of immunogens to skip nonneutralizing responses. ca_ES
dc.format application/pdf
dc.format.extent 12 p. ca_ES
dc.language.iso eng ca_ES
dc.publisher Biomed central ca_ES
dc.rights Aquest document està subjecte a aquesta llicència Creative Commons ca_ES
dc.rights.uri http://creativecommons.org/licenses/by/3.0/es/ ca_ES
dc.subject.other Sida -- Tractament ca_ES
dc.title Anti-MPER antibodies with heterogeneous neutralization capacity are detectable in most untreated HIV-1 infected individuals ca_ES
dc.type info:eu-repo/semantics/article ca_ES
dc.relation.publisherversion http://www.retrovirology.com/content/11/1/44
dc.rights.accesRights info:eu-repo/semantics/openAccess ca_ES
dc.type.version info:eu-repo/publishedVersion ca_ES
dc.indexacio Indexat a WOS/JCR ca_ES

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